An improved protocol for carrot haploid and doubled haploid plant production using induced parthenogenesis and ovule excision in vitro
نویسندگان
چکیده
منابع مشابه
Efficient haploid and doubled haploid production from unfertilized ovule culture of gentians (Gentiana spp.)
Factors affecting reliable plant regeneration from unfertilized ovule culture of gentians (Gentiana spp.) were examined. Cold pretreatment (4°C) of flower buds enhanced or maintained production of embryo-like structure (ELS). When 43 genotypes were surveyed in two different labs, 40 of them produced ELSs ranging from 0.01 to 26.5 ELSs per flower bud. No ELSs could be obtained in three genotypes...
متن کاملColchicine induced embryogenesis and doubled haploid production in maize (Zea mays L.) anther culture
This study involves in vitro androgenesis of <sp...
متن کاملProduction of doubled haploid plants of carnation (Dianthus caryophyllus L.) by pseudofertilized ovule culture
Doubled haploids of carnation (Dianthus caryophyllus L.) were obtained by pseudofertilized ovule culture. Emasculated ̄ower buds of carnation were pollinated with pollen inactivated by Xray irradiation. After 2±3 weeks, the ovaries were explanted and were cultured on solid MS medium containing 2 mM NAA, 2 mM BAP and 6% sucrose. Regenerated plants(R0) were morphologically different from the moth...
متن کاملHaploid Production in Higher Plant
Majority of higher plants are outbreeding, highly heterozygous and undergo a long developmental period before reaching their reproductive stage. Traditional breeding and crosspollinating procedures are both unpredictable and time-consuming. Haploids are plants with a gametophytic chromosome number and doubled haploids are haploids that have undergone chromosome duplication, represent a particul...
متن کاملCharacterization of in vitro haploid and doubled haploid Chrysanthemum morifolium plants via unfertilized ovule culture for phenotypical traits and DNA methylation pattern
Chrysanthemum is one of important ornamental species in the world. Its highly heterozygous state complicates molecular analysis, so it is of interest to derive haploid forms. A total of 2579 non-fertilized chrysanthemum ovules pollinated by Argyranthemum frutescens were cultured in vitro to isolate haploid progeny. One single regenerant emerged from each of three of the 105 calli produced. Chro...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: In Vitro Cellular & Developmental Biology - Plant
سال: 2014
ISSN: 1054-5476,1475-2689
DOI: 10.1007/s11627-014-9597-1